Calculate the Correct MOTS-c Dose: Reconstitution, Units, Timing, and Cycle

Perfect B - Blog - MOTS-c Dosage Calculator - Clinical vial with insulin syringe and bacteriostatic water
Valeria Marulanda

Valeria Marulanda

Valeria Marulanda is a board-certified Family Nurse Practitioner (FNP-BC) with a Bachelor of Science in Nursing from Florida Atlantic University and a Master of Science in Nursing from St. Thomas University. Since 2018, she has specialized in medical aesthetics, focusing on face and body treatments. Valeria loves longevity, science-driven skin treatments, and regenerating the human body from the inside out.

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Perfect B Aesthetic Medicine | 06.24.26 | 4 min read.

This article is for educational purposes only and does not constitute medical advice. Peptide therapy requires evaluation and prescription by a licensed medical provider. Do not use this information to self-administer or self-diagnose. Contact Perfect B at (786) 502-2260 to schedule a clinical evaluation.

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Perfect B · Clinical Protocols
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For the exact dosing protocol our patients follow, check the full guide.
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How Do You Reconstitute MOTS-c?

Use the volume the calculator returns above and add bacteriostatic water to your vial based on its size.

  • If your vial is 2 mg, add 1 ml of bacteriostatic water.
  • If your vial is 5 mg, add 2.5 ml of bacteriostatic water.
  • If your vial is 10 mg, add 5 ml of bacteriostatic water.
  • Once reconstituted, store the vial refrigerated and use within the active 20 day protocol window.

What Is the Concentration of MOTS-c in Your Vial?

Concentration is held constant across every vial size so your unit count never changes.

  • 2 mg vial reconstituted with 1 ml BAC water = 0.02 mg per unit.
  • 5 mg vial reconstituted with 2.5 ml BAC water = 0.02 mg per unit.
  • 10 mg vial reconstituted with 5 ml BAC water = 0.02 mg per unit.
  • One unit on a 1 ml insulin syringe always equals 0.02 mg of MOTS-c regardless of vial size.

How Many Units of MOTS-c Do You Inject Per Day?

Draw to the unit line on your insulin syringe, not to a milligram figure.

  • Dose per injection: 25 units on a 1 ml insulin syringe (equal to 0.5 mg of MOTS-c).
  • Total injections: 4 injections across the 20 day protocol, one every 5 days.
  • Total mg per cycle: 4 injections x 0.5 mg = 2 mg of MOTS-c per cycle.
  • Starting point: dose is fixed at 25 units from the first injection. No titration phase.
Perfect B - Blog - MOTS-c Dosage Calculator - Reconstitution with bacteriostatic water and insulin syringe
Draw to the unit line on a 1 ml insulin syringe. 1 unit equals 0.02 mg of MOTS-c at the standard reconstitution.

When Do You Inject MOTS-c?

Inject on the schedule the calculator returns above.

  • Injection site: subcutaneous, abdomen.
  • Timing: at night.
  • Frequency: one injection every 5 days, 4 injections total over 20 days.
  • Site rotation: rotate the exact spot within the same abdominal area each session.

What Is the Cycle for MOTS-c?

MOTS-c is a cycled protocol, not a daily-for-life peptide.

  • On phase: 20 day protocol (4 injections, one every 5 days).
  • Off phase: 4 months pause.
  • Repeat: patients typically run 2 full cycles per year on this protocol.
  • Why the off phase matters: MOTS-c works on mitochondrial signaling. Continuous dosing past the 20 day window blunts that signal. The 4 month pause is not optional.

Clinical tip: days between injections are rest days. If you miss a day, apply when you can and count 5 days from there.

Want the Same Protocol Built Around Your Goals?

Perfect B Aesthetic Medicine’s peptide protocol tool is built on real clinical data from 2,000+ patients treated in Florida.

Answer 6 questions and see what patients with similar goals are running: which peptides they use, typical dosing, injection schedule, reconstitution steps, cycle length, and when they pause.

See patient protocols at peptides.perfectb.com

Why Calculate Your MOTS-c Dose at Perfect B Aesthetic Medicine and Not at Home

A calculator gives you the numbers. A clinic gives you the answer to whether those numbers are safe for you. The protocol behind the tool above runs the same way for every patient at Perfect B Aesthetic Medicine, and that is what separates a supervised cycle from a guess at the kitchen counter.

  • Pharmacy-grade sourcing: your MOTS-c is dispensed through a licensed compounding pharmacy. Identity, purity, and sterility are documented. Grey-market online vials carry none of those guarantees.
  • Pre-protocol screening: labs, medical history, and contraindications are reviewed before a single vial is prescribed. Active cancer, pregnancy, bleeding disorders, and uncontrolled diabetes change the answer.
  • Measured response: you do not guess if MOTS-c is working. You measure response markers and any side effects across each cycle. The protocol can be adjusted when data justifies it.
  • Reconstitution done right: a mistake at the BAC water step changes your dose by an order of magnitude. The clinic walks you through the first reconstitution so the math is correct from injection one.
  • Real follow-up: the 20 day on, 4 months off cycle is monitored. If the protocol is not delivering, the clinic catches that early instead of two cycles later.
  • Florida-based clinical context: Perfect B Aesthetic Medicine is the clinic with the most peptide patients seen in Florida. That volume means every parameter in the calculator has been pressure-tested across thousands of real cycles.

You can run the calculator at home. You should not run the protocol at home. The numbers are accurate, but they are only safe inside a clinical relationship that screens you, supervises you, and adjusts when your physiology calls for it.

Frequently Asked Questions

1. What is the correct MOTS-c dose for you?

25 units per injection on a 1 ml insulin syringe, equal to 0.5 mg per injection. One injection every 5 days for a total of 4 injections over 20 days.

2. How do you reconstitute your MOTS-c vial?

Add 1 ml of bacteriostatic water for a 2 mg vial, 2.5 ml for a 5 mg vial, 5 ml for a 10 mg vial. Concentration stays at 0.02 mg per unit.

3. Where do you inject MOTS-c?

Subcutaneous, in the abdomen, at night. Rotate the exact spot within the same area each session.

4. Is this calculator a substitute for a consultation?

No. It surfaces the parameters the supervised protocol uses. The protocol itself starts with a medical evaluation, contraindication screening, and a written prescription from Perfect B Aesthetic Medicine.

  • 📍 Visit us at Perfect B Aesthetic Medicine | 3905 NW 107th Ave, Suite 104, Doral FL 33178
  • 📞 Call or message us at (786) 502-2260
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Book your peptide consultation at Perfect B Aesthetic Medicine today.

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